Connect the electrodes to the EVOM2 and leave the power off.3. Clean, dry and store the electrode.įor Voltage Measurements:1. To obtain the actual tissue reading, subtract theīlank resistance value.8. Measure the blank resistance and record the value.7. Precondition the electrode in growth media.5. Disconnect the EVOM2 from the charge and turn the Power on (I).2. QUICK REFERENCEFor complete instructions on performing measurements with the EVOM2, see "OPERATING INSTRUCTIONS" on page 5įor Resistance Measurements:1. Doing so provides for the maximum electrical isolation and reduces the noise to ensure readings of the highest accuracy.įor automation of TEER measurement, consider WPI’s REMS AutoSampler (WPI # REMS), which includes the robotic sampler, data acquisition board, base plate, Windows® based software and an electrode for either the 24- or 96-well plate. While the meter operates safely when it is plugged into an AC power source, WPI recommends disconnecting the meter from the charger when it is in use. When the battery power falls below the nominal threshold, the meter automatically powers off. A fully-charged battery pack will provide about eight hours of running time. ![]() The unit is supplied with an external battery charger. In addition, resistance readings are unaffected by membrane capacitance and membrane voltage.ĮVOM2 is designed with an internal rechargeable 6V NiMH 2700mAH battery pack. The EVOM2 produces an AC current that avoids electrode metal deposits and adverse effects on tissues, which can otherwise be caused by a DC current. When combined with WPI’s Endohm chamber, the EVOM2 can also be used to perform trans endothelial electrical resistance measurement. The EVOM2 qualitatively measures cell monolayer health and quantitatively measures cell confluence. The confluence of the cellular monolayer is determined by an increase in TEER detected using the unique electronic circuit of the EVOM2 and STX2 electrode (included with the instrument). INTRODUCTIONEVOM was the first instrument designed to perform routine Trans Epithelial Electrical Resistance (TEER) measurement in tissue culture research. 1–The EVOM2 measures resistance or voltage across a tissue membrane. NOTES and TIPS contain helpful information.įig. Warnings alert you to actions that can cause personal injury or pose a physical threat. Cautions warn against actions that can cause damage to equipment. No part of this publication may be reproduced or translated into any language, in any form, without prior written permission of World Precision Instruments, Inc.ĪBOUT THIS MANUALThe following symbols are used in this guide: 20Ĭopyright © 2016 by World Precision Instruments, Inc. ![]() 20APPENDIX C: IMPROVING THE ACCURACY AND REPEATABILITY OF THE SYSTEM. 19ĪPPENDIX B: 24 MM DIAMETER (6-WELL) INSERTS AND STX ELECTRODES. 18Resistance Value of the “Blank” Insert.
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